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通过钝化global regulator FruR调整大肠杆菌中碳流量来提高色氨酸的产量

发布日期:2016.11.30 浏览次数(659)

大肠杆菌中,果糖抑制剂(FruR)通过中心代谢途径影响碳的流量。这篇研究中,FB-04大肠杆菌通过敲除了FruR基因从而使FruR失活使色氨酸的产量增加。基因敲除的FB-04大肠杆菌(△fruR)不仅展现出更高的生长效率而且还大幅度提升了色氨酸的产量。FB-04(△FruR)大肠杆菌中色氨酸的产量和每分子葡萄糖产生的色氨酸相比于没有敲除的大肠杆菌分别增加了65.5%和52.4%。代谢组学分析,显示基因敲除的大肠杆菌显著提高了糖酵解、戊糖磷酸途径和三羧酸循环代谢,用于生物合成色氨酸的前体和基质水平升高。在不考虑遗传背景下,fruR的缺失会升高色氨酸的产量并提高碳源的利用效率。

这篇文章中使用的代谢组学技术谱领生物已开发成熟、完善,并对外提供服务,已为多位客户提供服务并获得好评。

 

2016-江南大学-吴敬-刘丽娜-paper1_页面_3.png

文献内容


Title:Modulating the direction of carbon flow in Escherichia coli to improve 1-tryptophan production by inactivating the global regulator FruR

Author:Lina Liua,b, Xuguo Duana,b, Jing Wua,b,∗

Abstract:
The fructose repressor (FruR) affects carbon flux through the central metabolic pathways of Escherichia coli. In this study, l-tryptophan production in Escherichia coli FB-04 was improved by knocking out the fruR gene, thereby inactivating FruR. This fruR knockout strain, E. coli FB-04(L1fruR), not only exhibited higher growth efficiency, it also showed substantially improved l-tryptophan production. l-tryptophan production by E. coli FB-04(L1fruR) and l-tryptophan yield per glucose were increased by 62.5% and 52.4%, respectively, compared with the parent E. coli FB-04. Metabolomics analysis showed that the fruR knockout significantly enhances metabolic flow through glycolysis, the pentose phosphate pathway and the TCA cycle, increasing levels of critical precursors and substrates for l-tryptophan biosynthesis. These results indicate that fruR deletion should enhance l-tryptophan production and improve the efficiency of carbon source utilization independent of genetic background.

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