氨基酸定量

包括必需和非必需氨基酸在内的二十种常见的氨基酸和氨基酸衍生物等近百种氨基酸。

]氨基酸定量

氨基酸(Amino Acid)是生命体的基本物质之一,是含有碱性氨基和酸性羧基的有机化合物,是组成酶和蛋白质的基本单位,作为生物小分子,氨基酸对生理功能和临床诊断具有重要作用。氨基酸在机体内总体来说可发挥以下作用:① 合成组织蛋白质;② 变成酸、激素、抗体、肌酸等含氨物质;③ 转变为碳水化合物和脂肪;④ 氧化成二氧化碳和水及尿素,产生能量。

根据侧链R基团上的结构不同,常见的氨基酸有20种。根据营养学分类,又分为必需氨基酸、半必需氨基酸和条件必需氨基酸。除此之外,还有近百种氨基酸的衍生物,不同的氨基酸及其衍生物具有不同的营养价值和生理作用,在机体内发挥不同的作用。如果人体缺乏任何一种氨基酸,就可能导致生理功能异常,影响抗体代谢的正常进行,最后导致疾病。精氨酸和瓜氨酸对尿素形成十分重要;胱氨酸摄入不足会引起胰岛素减少,血糖升高等。氨基酸的存在不仅提供蛋白合成的重要原料,而且对于促进生长,进行正常代谢、维持生命提供了物质基础。如果缺少了其中的某一种,生物体的正常代谢就会受到障碍,甚至导致各种疾病的发生或生命活动的终止。

]服务流程

图片关键词


]样本量要求

图片关键词

]生物学重复

相较于其他组学,代谢组学更加灵敏,更接近于真实情况。因此,为保证实验结果的可靠性,要求更多的生物学样本重复。我们建议:

临床样本,单组不少于30 例重复;

动物样本,单组不少于9 例重复;

细胞、微生物样本,单组不少于5 例重复;

其他如植物样本,单组不少于7 例重复。

]仪器平台



平台一:超高效液相色谱串联质谱联用仪 1290-6470( Agilent UHPLC-MS/MS)


图片关键词

灵敏度更高 — 低至亚飞克级的检测限更清晰的信号和更低的噪音 — 安捷伦喷射流技术采用超热氮气

改善离子生成与脱溶剂。可灵活升级为 Agilent iFunnel 技术的安全投资通过弯曲的圆锥形六极杆创新组

件进行产物离子的高效采集和传输筛查、确证和定量 — 触发式多反应监测 (tMRM) 结合了快速、灵敏

的 MRM 定量分析与产物离子谱图,可用于谱库检索、化合物筛查和确证。



平台二:超高效液相色谱串联质谱联用仪 Triple Quad® 5500 系统 ( Waters UPLC-AB Sciex MS/MS)

仪器2.jpgACQUITY UPLC 系统不断为世界各地的实验室设立新的基准。UPLC 分离度和灵敏度使科学家们能够鉴

定出复杂生物样本中按量极低的化合物。Triple Quad 5500 LC-MS/MS 系统专为提供高级别灵敏度和耐

用性而设计,即便面对最复杂、要求最严苛的基质也游刃有余。在环境、靶向定量、临床研究、食品和

饮料应用所需的多组分定量分析方面表现卓越。



平台三:超高效液相色谱高分辨质谱联用仪 Q Exactive ™ ( Thermo Scientific Orbitrap MS)

图片关键词搭配 UltiMate ™ 3000 系统,使用 Q Exactive ™ 组合型四极杆 Orbitrap 质谱仪可以快速可靠地识别、

定量和确认更多化合物。 本台式 UHPLC-HRMS 系统将四极杆母离子选择性与高分辨率和准确质量数

(HRAM)Orbitrap 检测相结合,提供出色性能和多功能性。 Q Exactive 质谱仪特别适用于非目标或目

标化合物筛查,也能够实现广泛的定性和定量应用。



平台四:气相色谱质谱联用仪(GC-MS)7890A/5975C(Agilent GC-MS)

图片关键词气相色谱 - 质谱与生俱来的定量准确性 ( 无液相色谱 - 质谱联用平台常用的电喷雾离子化所存在的基质

抑制效应 )、超高的稳定性和易用性,以及不俗的灵敏度和高通量,能胜任多数代谢组学和基础生命科

学研究任务。尤其是那些浓度较高、具有挥发性的物质或虽具有亲水性但衍生后具有挥发性的代谢物,

如脂肪酸、糖酵解和三羧酸循环相关代谢物、氨基酸和中长链脂肪酸等,都十分适用。



]服务周期

样本检测:15-25 个工作日;

数据分析:5-15 个工作日;

服务周期自收到预付款、待测样本和客户确认检测要求之日起计算。


]应用方向

表型组和生理功能研究;

临床早期预测、诊疗研究;

病理学研究 ;

天然药物和药理研究 ;

中医现代化和理论研究;

食品科学、安全和营养学研究;

畜牧业和农林业研究;

植物学和环境研究;

毒理学研究。




定量物质


NoMetabolite中文名CAS号ClassHMDB ID
1Glycine甘氨酸56-40-6Amino acids, peptides, and analoguesHMDB0000123
2L-AlanineL-丙氨酸56-41-7Amino acids, peptides, and analoguesHMDB0000161
3L-ArginineL-精氨酸74-79-3Amino acids, peptides, and analoguesHMDB0000517
4L-AsparagineL-天冬酰胺70-47-3Amino acids, peptides, and analoguesHMDB0000168
5L-Aspartic acidL-天冬氨酸56-84-8Amino acids, peptides, and analoguesHMDB0000191
6L-CysteineL-半胱氨酸52-90-4Amino acids, peptides, and analoguesHMDB0000574
7L-CystineL-胱氨酸56-89-3Amino acids, peptides, and analoguesHMDB0000192
8L-Glutamic acidL-谷氨酸56-86-0Amino acids, peptides, and analoguesHMDB0000148
9L-GlutamineL-谷氨酰胺56-85-9Amino acids, peptides, and analoguesHMDB0000641
10L-HistidineL-组氨酸71-00-1Amino acids, peptides, and analoguesHMDB0000177
11L-IsoleucineL-异亮氨酸73-32-5Amino acids, peptides, and analoguesHMDB0000172
12L-LeucineL-亮氨酸61-90-5Amino acids, peptides, and analoguesHMDB0000687
13L-LysineL-赖氨酸56-87-1Amino acids, peptides, and analoguesHMDB0000182
14L-MethionineL-蛋氨酸63-68-3Amino acids, peptides, and analoguesHMDB0000696
15L-OrnithineL-鸟氨酸3184-13-2Amino acids, peptides, and analoguesHMDB0000214
16L-PhenylalanineL-苯丙氨酸63-91-2Amino acids, peptides, and analoguesHMDB0000159
17L-ProlineL-脯氨酸147-85-3Amino acids, peptides, and analoguesHMDB0000162
18L-SerineL-丝氨酸56-45-1Amino acids, peptides, and analoguesHMDB0000187
19L-ThreonineL-苏氨酸72-19-5Amino acids, peptides, and analoguesHMDB0000167
20L-TryptophanL-色氨酸73-22-3Amino acids, peptides, and analoguesHMDB0000929
21L-TyrosineL-酪氨酸60-18-4Amino acids, peptides, and analoguesHMDB0000158
22L-ValineL-缬氨酸72-18-4Amino acids, peptides, and analoguesHMDB0000883
23L-NorleucineL-正亮氨酸327-57-1Amino acids, peptides, and analoguesHMDB0001645
24L-NorvalineL-正缬氨酸760-78-1Amino acids, peptides, and analoguesHMDB0013716
25L-Pipecolic acidL-高脯氨酸3105-95-1Amino acids, peptides, and analoguesHMDB0000716
26L-KynurenineL-犬尿氨酸2922-83-0Amino acids, peptides, and analoguesHMDB0000684
27L-Allo-isoleucineL-别异亮氨酸1509-34-8Amino acids, peptides, and analoguesHMDB0000557
28L-HomocysteineL-同型半胱氨酸6027-13-0Amino acids, peptides, and analoguesHMDB0000742
29L-HomocystineL-同型胱氨酸626-72-2Amino acids, peptides, and analoguesHMDB0000676
30L-HomoserineL-同型丝氨酸672-15-1Amino acids, peptides, and analoguesHMDB0000719
31L-ThyronineL-甲状腺素1596-67-4Amino acids, peptides, and analoguesHMDB0000667
321-Methyl-L-histidine1-甲基组氨酸332-80-9Amino acids, peptides, and analoguesHMDB0000001
333-Methyl-L-histidine3-甲基组氨酸368-16-1Amino acids, peptides, and analoguesHMDB0000479
342-aminobutyric acid2-氨基丁酸1492-24-6Amino acids, peptides, and analoguesHMDB0000452
352-Aminoisobutyric acid2-氨基异丁酸62-57-7Amino acids, peptides, and analoguesHMDB0001906
363-Aminobutyric acid3-氨基丁酸541-48-0Amino acids, peptides, and analoguesHMDB0031654
373-Aminoisobutyric acid3-氨基异丁酸4249-19-8Amino acids, peptides, and analoguesHMDB0002166
38Gamma-Aminobutyric acidγ-氨基丁酸56-12-2Amino acids, peptides, and analoguesHMDB0000112
392,3-Diaminopropionic acid2,3-二氨基丙酸4033-39-0Amino acids, peptides, and analoguesHMDB0002006
402-Aminoadipic acid2-氨基乙二酸1118-90-7Amino acids, peptides, and analoguesHMDB0000510
413-Chlorotyrosine3-氯酪氨酸7423-93-0Amino acids, peptides, and analoguesHMDB0001885
422-Phenylglycine2-苯基甘氨酸2835-06-5Amino acids, peptides, and analoguesHMDB0002210
433-Methylcrotonylglycine3-甲基巴豆酰甘氨酸33008-07-0Amino acids, peptides, and analoguesHMDB0000459
443-Nitrotyrosine3-硝基酪氨酸3604-79-3Amino acids, peptides, and analoguesHMDB0001904
454-Acetamidobutyric acidN-乙酰-4-氨基丁酸3025-96-5Amino acids, peptides, and analoguesHMDB0003681
464-Amino-3-hydroxybutyric acid4-氨基-3-氨基丁酸924-49-2Amino acids, peptides, and analoguesHMDB0061877
474-Hydroxyphenylglycine对羟基苯甘氨酸938-97-6Amino acids, peptides, and analoguesHMDB0244973
484-Hydroxyproline4-羟基脯氨酸51-35-4Amino acids, peptides, and analoguesHMDB0000725
495-Aminolevulinic acid5-氨基乙酰丙酸106-60-5Amino acids, peptides, and analoguesHMDB0001149
505-Aminopentanoic acid5-氨基戊酸660-88-8Amino acids, peptides, and analoguesHMDB0003355
515-Hydroxytryptophan5-羟基色氨酸4350-09-8Amino acids, peptides, and analoguesHMDB0000472
525-Hydroxylysine5-羟基赖氨酸1190-94-9Amino acids, peptides, and analoguesHMDB0000450
536-Aminocaproic acid6-氨基己酸60-32-2Amino acids, peptides, and analoguesHMDB0001901
54Asymmetric dimethylarginine不对称二甲基精氨酸30315-93-6Amino acids, peptides, and analoguesHMDB0001539
55Symmetric dimethylarginine对称二甲基精氨酸30344-00-4Amino acids, peptides, and analoguesHMDB0003334
56Beta-Alanineβ-丙氨酸107-95-9Amino acids, peptides, and analoguesHMDB0000056
57Pyroglutamic acid焦谷氨酸98-79-3Amino acids, peptides, and analoguesHMDB0000267
58Citrulline瓜氨酸372-75-8Amino acids, peptides, and analoguesHMDB0000904
59Creatine肌酸57-00-1Amino acids, peptides, and analoguesHMDB0000064
60Cysteinylglycine半胱氨酰甘氨酸19246-18-5Amino acids, peptides, and analoguesHMDB0000078
61Guanidoacetic acid胍基乙酸352-97-6Amino acids, peptides, and analoguesHMDB0000128
62Homocitrulline高瓜氨酸1190-49-4Amino acids, peptides, and analoguesHMDB0000679
63Homoarginine高精氨酸156-86-5Amino acids, peptides, and analoguesHMDB0000670
64Argininosuccinic acid精氨酰琥珀酸2387-71-5Amino acids, peptides, and analoguesHMDB0000052
65Glycylproline甘氨酰脯氨酸704-15-4Amino acids, peptides, and analoguesHMDB0000721
66Glycyl-L-leucine甘氨酰亮氨酸869-19-2Amino acids, peptides, and analoguesHMDB0000759
67Carnosine肌肽305-84-0Amino acids, peptides, and analoguesHMDB0000033
68Anserine鹅肌肽584-85-0Amino acids, peptides, and analoguesHMDB0000194
69Cystathionine胱硫醚56-88-2Amino acids, peptides, and analoguesHMDB0000099
70Glycyl-glycine双甘肽556-50-3Amino acids, peptides, and analoguesHMDB0011733
71O-AcetylserineO-乙酰丝氨酸5147-00-2Amino acids, peptides, and analoguesHMDB0003011
72Phenylacetylglutamine苯乙酰谷氨酰胺28047-15-6Amino acids, peptides, and analoguesHMDB0006344
73Phenylacetylglycine苯乙酰甘氨酸500-98-1Amino acids, peptides, and analoguesHMDB0000821
74Phosphoserine磷酸丝氨酸407-41-0Amino acids, peptides, and analoguesHMDB0000272
75S-AdenosylhomocysteineS-腺苷同型半胱氨酸979-92-0Amino acids, peptides, and analoguesHMDB0000939
76S-AdenosylmethionineS-腺苷蛋氨酸485-80-3Amino acids, peptides, and analoguesHMDB0001185
77Sarcosine肌氨酸107-97-1Amino acids, peptides, and analoguesHMDB0000271
78S-CarboxymethylcysteineS-羧甲基半胱氨酸2387-59-9Amino acids, peptides, and analoguesHMDB0029415
79MethylcysteineS-甲基-L-半胱氨酸1187-84-4Amino acids, peptides, and analoguesHMDB0002108
80gamma-Glutamylalanineγ-谷氨酰丙氨酸5875-41-2Amino acids, peptides, and analoguesHMDB0006248
81gamma-Glutamylglutamineγ-谷氨酰谷氨酰胺10148-81-9Amino acids, peptides, and analoguesHMDB0011738
82N-Acetylaspartylglutamic acidN-乙酰天冬氨酰谷氨酸3106-85-2Amino acids, peptides, and analoguesHMDB0001067
83Phenylalanylphenylalanine苯丙氨酰苯丙氨酸2577-40-4Amino acids, peptides, and analoguesHMDB0013302
84Dimethylglycine二甲基甘氨酸1118-68-9Amino acids, peptides, and analoguesHMDB0000092
85N-AcetylarginineN-乙酰精氨酸155-84-0Amino acids, peptides, and analoguesHMDB0004620
86N2-AcetylornithineN-乙酰鸟氨酸6205-08-9Amino acids, peptides, and analoguesHMDB0003357
87AcetylglycineN-乙酰甘氨酸543-24-8Amino acids, peptides, and analoguesHMDB0000532
88N-AcetylalanineN-乙酰丙氨酸97-69-8Amino acids, peptides, and analoguesHMDB0000766
89N-Acetylaspartic acidN-乙酰天冬氨酸997-55-7Amino acids, peptides, and analoguesHMDB0000812
90N-AcetylcysteineN-乙酰半胱氨酸616-91-1Amino acids, peptides, and analoguesHMDB0001890
91N-Acetylglutamic acidN-乙酰谷氨酸1188-37-0Amino acids, peptides, and analoguesHMDB0001138
92N-AcetylglutamineN-乙酰谷氨酰胺2490-97-3Amino acids, peptides, and analoguesHMDB0006029
93N-AcetylhistidineN-乙酰组氨酸2497-02-1Amino acids, peptides, and analoguesHMDB0032055
94N-AcetylisoleucineN-乙酰异亮氨酸3077-46-1Amino acids, peptides, and analoguesHMDB0061684
95N-AcetylleucineN-乙酰亮氨酸1188-21-2Amino acids, peptides, and analoguesHMDB0011756
96N-AcetylmethionineN-乙酰蛋氨酸65-82-7Amino acids, peptides, and analoguesHMDB0011745
97N-AcetylphenylalanineN-乙酰苯丙氨酸2018-61-3Amino acids, peptides, and analoguesHMDB0000512
98N-AcetylprolineN-乙酰脯氨酸68-95-1Amino acids, peptides, and analoguesHMDB0094701
99N-AcetyltryptophanN-乙酰色氨酸1218-34-4Amino acids, peptides, and analoguesHMDB0013713
100N-AcetyltyrosineN-乙酰酪氨酸537-55-3Amino acids, peptides, and analoguesHMDB0000866
101N-AcetylvalineN-乙酰缬氨酸96-81-1Amino acids, peptides, and analoguesHMDB0011757
102N-AcetyllysineN-乙酰赖氨酸1946-82-3Amino acids, peptides, and analoguesHMDB0000446
103N-AcetylcitrullineN-乙酰瓜氨酸33965-42-3Amino acids, peptides, and analoguesHMDB0000856
104N6-AcetyllysineN6-乙酰赖氨酸692-04-6Amino acids, peptides, and analoguesHMDB0000206
105Ureidopropionic acid3-酰脲丙酸462-88-4Amino acids, peptides, and analoguesHMDB0000026
106Ureidosuccinic acid脲基琥珀酸13184-27-5Amino acids, peptides, and analoguesHMDB0000828
107CinnamoylglycineN-肉桂酰甘氨酸16534-24-0Amino acids, peptides, and analoguesHMDB0011621
108FormylglycineN-甲酰甘氨酸2491-15-8Amino acids, peptides, and analoguesHMDB0255145
109IsobutyrylglycineN-异丁酰甘氨酸15926-18-8Amino acids, peptides, and analoguesHMDB0000730
110IsovalerylglycineN-异戊酰甘氨酸16284-60-9Amino acids, peptides, and analoguesHMDB0000678
111N-PhenylacetylphenylalanineN-苯乙酰苯丙氨酸738-75-0Amino acids, peptides, and analoguesHMDB0002372
112TiglylglycineN-巴豆酰基甘氨酸35842-45-6Amino acids, peptides, and analoguesHMDB0000959
113Valylglycine缬氨酰甘氨酸686-43-1Amino acids, peptides, and analoguesHMDB0029127
1143-Iodo-L-tyrosine3-碘- -酪氨酸70-78-0Amino acids, peptides, and analoguesHMDB0000021
1153,5-Diiodo-L-tyrosine3,5-二碘-L-酪氨酸300-39-0Amino acids, peptides, and analoguesHMDB0003474
116Thyroxine甲状腺素51-48-9Amino acids, peptides, and analoguesHMDB0000248



服务示例





图片关键词

氨基酸提取离子色谱图(XIC)

图片关键词

部分氨基酸标准曲线参考





文献案例


  • Chen GH, Yang DY, Xie P, et al. Amino acid metabolic dysfunction revealed in the prefrontal cortex of a rat model of depression. Behavioural Brain Research. 2014: 286-292.

  • Sanchez-Hernandez L, Marina ML. A capillary electrophoresis-tandem mass spectrometry methodology for the determination of non-protein amino acids in vegetable oils as novel markers for the detection of adulterations in olive oils. J Chromatogr A, 2011, 1218(30): 4944-4951.



微信公众号或添加客服号