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游离不饱和脂肪酸描绘肥胖个体代谢状态的研究

发布日期:2016.10.13 浏览次数(318)

肥胖,常常是心血管疾病、2型糖尿病等代谢性疾病的重要病因,本篇文献研究发现体重正常的健康者和健康的肥胖者血清中的游离脂肪酸谱非常相似,但是患有糖尿病的肥胖者的血清样本中游离脂肪酸显著性升高,其中不饱和脂肪酸更为突出。又以C20:3n-6最为显著。在后期10年随访样本收集研究中发现那些后来发现糖尿病病变的肥胖者的不饱和脂肪酸发生了显著性变化,这些变化远远早于血糖和胰岛素等目前临床指标的变化。在接受代谢手术治疗后,这些指标明细降低,并且,术前的不饱和脂肪酸水平的水平可以预测术后2年内糖尿病的复发情况。

文章中提到的技术难点是游离脂肪酸含量较低且数量较多,如何一次性同时检测、绝对定量这些游离脂肪酸是需要解决的重要问题之一,谱领生物已开发成熟、完善的,从C2到C24包括三十多种短链、中长链游离脂肪酸的绝对定量方案,并对外提供服务,已为多位客户提供服务并获得好评。详情请见脂肪酸检测分析
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文献内容


Title:Circulating Unsaturated Fatty Acids Delineate the Metabolic Status of Obese Individuals.

Author:Yan Ni, Linjing Zhao, Haoyong Yu, Xiaojing Ma, Yuqian Bao, Cynthia Rajani, Lenora W.M. Loo, Yurii B. Shvetsov, Herbert Yu, Tianlu Chen, Yinan Zhang, Congrong Wang, Cheng Hu, Mingming Su, Guoxiang Xie, Aihua Zhaoa Wei Jia, and Weiping Jia

Journal: EBioMedicine. 2015 Oct; 2(10): 1513–1522.

Keywords: Free fatty acids, Metabolic syndrome, Obesity, Unsaturated fatty acids, Type 2 diabetes, Insulin resistance.

Abstract:

Background

Obesity is not a homogeneous condition across individuals since about 25–40% of obese individuals can maintain healthy status with no apparent signs of metabolic complications. The simple anthropometric measure of body mass index does not always reflect the biological effects of excessive body fat on health, thus additional molecular characterizations of obese phenotypes are needed to assess the risk of developing subsequent metabolic conditions at an individual level.

Methods

To better understand the associations of free fatty acids (FFAs) with metabolic phenotypes of obesity, we applied a targeted metabolomics approach to measure 40 serum FFAs from 452 individuals who participated in four independent studies, using an ultra-performance liquid chromatograph coupled to a Xevo G2 quadruple time-of-flight mass spectrometer.

Findings

FFA levels were significantly elevated in overweight/obese subjects with diabetes compared to their healthy counterparts. We identified a group of unsaturated fatty acids (UFAs) that are closely correlated with metabolic status in two groups of obese individuals who underwent weight loss intervention and can predict the recurrence of diabetes at two years after metabolic surgery. Two UFAs, dihomo-gamma-linolenic acid and palmitoleic acid, were also able to predict the future development of metabolic syndrome (MS) in a group of obese subjects.

Interpretation

These findings underscore the potential role of UFAs in the MS pathogenesis and also as important markers in predicting the risk of developing diabetes in obese individuals or diabetes remission after a metabolic surgery.

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